VIPR RNA-guided DNA recognition by noncontiguous geometric triplex formation.
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VIPR systems achieve precise DNA targeting through noncontiguous triplex formation, with 21 cryo-EM structures revealing the mechanism of target engagement.
- Why it matters: Understanding how VIPR proteins and vrRNA recognize specific DNA sequences addresses a key gap in programmable DNA targeting technologies, which could enhance gene regulation tools.
- What they did: Researchers used cryo-electron microscopy to determine 21 structures showing Vipr protomers forming a helical filament along vrRNA, facilitating GGY motif sequestration and NN base pairing, leading to a gapped DNA hybrid helix.
- The result: This triplex-mediated recognition mechanism enables noncontiguous, programmable RNA-guided DNA targeting, opening new possibilities for precise genetic interventions.