Proteome-wide profiling of lysine β-hydroxybutyrylation in Reynoutria japonica (Huzhang) and its dynamics under hydrogen sulfide stress.

Proteome-wide profiling reveals 17,102 lysine β-hydroxybutyrylation sites in Reynoutria japonica, linking Kbhb to plant stress response and metabolism regulation.

• Why it matters: Understanding how plants respond to environmental signals like hydrogen sulfide is crucial for improving stress resilience and metabolic adaptation, yet this area remains largely unexplored.
• What they did: The study employed proteome-wide analysis to identify Kbhb sites, examined changes under H2S stress, and validated regulatory roles of specific enzymes and modifications using pharmacological, genetic, and heterologous expression approaches.
• The result: Findings show Kbhb acts as a metabolic activator, especially on enzymes like IDH, and modulates chromatin-related responses, providing insights into epigenetic and metabolic adaptation mechanisms in plants under stress.