An expanded codebook of human transcription factor DNA-binding specificity.
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The expanded human transcription factor codebook reveals 130 new DNA-binding motifs for 177 TFs, enhancing understanding of gene regulation in the human genome.
- Why it matters: Deciphering TF binding motifs is crucial for understanding gene expression regulation, yet many human TFs lack characterized motifs, limiting insights into their functions and regulatory networks.
- What they did: The team conducted over 4,000 experiments using in vitro and in vivo assays to determine DNA-binding specificities for 332 poorly characterized human TFs, identifying motifs for 177 of them.
- The result: The findings significantly expand the known TF recognition vocabulary, with motifs enriched in cellular binding sites, enabling better prediction of gene expression and regulatory elements.