Hypatia: Comparative Isoform Profiling Across Cell Populations from Long-Read Single-Cell Transcriptomes

Hypatia enables systematic, isoform-level comparison across cell populations from long-read single-cell RNA-seq, with Cramer's V improving isoform-usage shift precision and Tsallis entropy refining isoform diversity.

• Why it matters: Long-read single-cell RNA-seq can quantify isoforms per cell, but methods to delineate cell population-specific isoforms across multiple isoform complexity dimensions are limited.
• What they did: Hypatia compares isoform usage, diversity, and expression, using Cramer's V for usage shifts and Tsallis entropy for isoform diversity, then integrates these analyses on real datasets.
• The result: Cramer's V improves precision for detecting isoform usage shifts but detects fewer events in sparser data, while Tsallis entropy improves comparability across genes and cells.