H(+)-Pyrophosphatase VP1 Enhances Low Phosphorus Tolerance by Interacting With the Auxin Influx Carrier AUX1 in Wheat.

Overexpression of EdVP1 from Elymus dahuricus increases wheat yield by over 14% under low-phosphorus conditions, improving phosphorus use efficiency and root architecture.

• Why it matters: Phosphorus deficiency limits wheat productivity globally, and understanding molecular mechanisms to enhance PUE is crucial for food security; the role of VP1-like genes in this process remains unclear.
• What they did: A VP1-like gene from Elymus dahuricus was introduced into wheat, and field trials, physiological, and molecular analyses were conducted, revealing improved growth, P accumulation, and stress responses.
• The result: The transgenic wheat lines showed increased auxin levels and upregulated TaAUX1, with direct interaction between TaVP1 and TaAUX1, suggesting a novel VP1-mediated pathway enhancing P deficiency tolerance via auxin signaling.