SNOOPPI: A Sequence-Normalized Database of On- and Off-Target Protein-Protein Interactions.

SNOOPPI catalogs over 33,600 positive and 5,300 negative protein-protein interactions, integrating isoform, post-translational modification, mutation, and binding site data.

• Why it matters: Current PPI databases lack comprehensive evidence-based negatives and detailed proteoform information, limiting their utility for accurate modeling and therapeutic development.
• What they did: The team curated SNOOPPI from the IntAct database, reclassifying interactions with experimental metadata to create a unified, sequence-aware dataset of binary PPIs.
• The result: SNOOPPI offers a high-confidence resource for sequence-based modeling, benchmarking, and designing novel protein interactions, while explicitly capturing gaps in current experimental knowledge.