Spatial proteomics identifies FXYD6 as a dual-site protein of neuromuscular junction in the diaphragm.
- Open access
Spatial proteomics reveals FXYD6 as a dual-site protein at the neuromuscular junction in the diaphragm, with significant enrichment in type IIa fibers.
- Why it matters: Understanding the molecular composition of the NMJ is crucial because these synapses are vulnerable in neuromuscular diseases, impacting respiratory function and muscle health.
- What they did: The study used spatial proteomics to analyze the diaphragm, identifying 115 proteins enriched at the NMJ and comparing them with myotendinous junction proteins, highlighting FXYD6's role.
- The result: Findings show FXYD6 interacts with Na+/K+-ATPase subunits, supporting ionic homeostasis and fatigue resistance, providing insights into synaptic regulation and potential therapeutic targets.