Toward a more precise immunophenotypic and molecular definition of human cMoPs.

Using CD115 instead of CLEC12A improves the precision of defining human common monocyte progenitors, revealing their molecular complexity.

• Why it matters: Accurate identification of cMoPs is essential for understanding monocyte development and immune responses, but previous markers provided incomplete or inconsistent definitions.
• What they did: The study employed flow cytometry, scRNA-seq, morphological, and functional analyses on human cMoPs, comparing cells defined by CLEC12A and CD115 markers, and identified five transcriptional features.
• The result: Findings show that gating based on CD115 yields a more accurate cMoP definition, uncovering diverse transcriptional programs and key transcription factors like IRF8, PU.1, and C/EBPβ, advancing knowledge of monocyte development.