Yeast Rad55-Rad57-SHU paralog complex dynamically promotes Rad51 filament formation.

Yeast Rad55-Rad57-SHU complex enhances Rad51 filament formation through dynamic structural and nucleotide-regulated mechanisms.

• Why it matters: Understanding how Rad51 filaments form is crucial for insights into DNA repair and genome stability, with implications for tumor suppression in humans.
• What they did: Using cryo-electron microscopy, six distinct states of the Saccharomyces cerevisiae Rad51 paralog complex were captured, revealing its role in recruiting Rad51 to single-stranded DNA and the regulatory influence of nucleotide binding.
• The result: The findings elucidate a dual-nucleotide mechanism controlling complex stability and release, offering a structural blueprint for Rad51 paralog function across eukaryotic species.