Vps1 drives membrane constriction and fission necessary for endosomal protein sorting.

Vps1 drives membrane constriction and fission for endosomal protein sorting, with mutants impairing GTP hydrolysis failing to achieve fission and causing cargo mis-sorting.

• Why it matters: Proper trafficking between endosomes and the Golgi is crucial for cellular function, and disruptions lead to metabolic and neurological disorders, highlighting the need to understand underlying mechanisms.
• What they did: Using cell-free reconstitution and live-cell assays, the study examined Vps1's role, revealing its assembly into scaffolds on membrane tubules and dependence on GTP hydrolysis for membrane fission.
• The result: Findings identify essential motifs for Vps1 function and show that loss of Vps1 activity causes broad mis-sorting of Golgi and plasma membrane proteins, confirming its mechanochemical role in membrane fission.