Cilia beating of ependymal cells regulates adult neural stem cell quiescence via mechanical forces mediated by PKD1/2-TRPM3.
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Cilia beating of ependymal cells maintains adult neural stem cell quiescence through PKD1/2-TRPM3-mediated mechanical forces, with brief inhibition activating stem cells in vivo.
- Why it matters: Understanding how mechanical forces influence stem cell behavior is crucial for insights into tissue maintenance and regeneration, yet their specific role remains unclear.
- What they did: The study used magnetic bead-coupled antibodies and magnetic fields to transiently inhibit ependymal cilia beating in vivo, combined with CRISPR-Cas9 gene editing and pharmacological methods to dissect the pathway.
- The result: Mechanical forces from cilia beating enforce NSC quiescence via PKD1/2 and TRPM3 calcium transients, and disrupting this process leads to rapid stem cell activation, highlighting a mechanosensitive regulation mechanism.