LipoID profiles lipid droplet interactions and identifies interorganelle regulators.
- 9 cites
LipoID uncovers mitochondrial voltage-dependent anion channel 3 as a key regulator of lipid droplet-mitochondria interactions, revealing new interorganellar regulation mechanisms.
- Why it matters: Understanding lipid droplet interactions with other organelles is crucial for insights into cellular metabolic homeostasis, but their transient nature has hindered detailed molecular profiling.
- What they did: The team developed LipoID, a small-molecule photocatalytic proximity labeling method, using LD-targeting probes to capture and analyze proteins interacting with lipid droplets via mass spectrometry.
- The result: LipoID successfully identified known LD biomarkers and discovered VDAC3 as a regulator of LD-mitochondria proximity, providing a platform for studying interorganellar regulation and lipid metabolism.