Design Tradeoffs in High Resolution, Large Field-of-View 3D All-Optical Control of Neural Circuits

Cellular-resolution holographic optogenetic control was demonstrated over neurons separated by more than 1.4 mm in a single hologram using scanless temporally focused multiphoton designs.

• Why it matters: Existing “one-size-fits-all” microscopes are not optimized for different optogenetic imaging paradigms, limiting accessible spatial range and resolution tradeoffs.
• What they did: The work compares microscope designs for scanless temporally focused holographic optogenetics, using optical-element and lens-order changes and newer high-NA, 2P-optimized low-magnification objectives.
• The result: The design framework and head-to-head comparisons show how optical changes can improve accessible range while maintaining resolution, enabling larger simultaneous neural manipulation in conventional microscopes.