Multiscale, Survey-and-Target Optical Super-Resolution Histology

MOSH (multiscale optical super-resolution histology) achieves ~twofold resolution enhancement while preserving tissue-scale context, enabling coordinate-guided 3D interrogation across cultured cells, sections, and unsectioned biopsies.

• Why it matters: Super-resolution microscopy is powerful for subcellular architecture, but histological screening needs a practical balance of resolution, axial access, imaging area, throughput, and instrument complexity.
• What they did: The platform integrates stationary multifocal excitation, continuous sample translation, tunable axial refocusing, and parallelized reconstruction in a shared spatial-coordinate framework, producing virtual H&E images.
• The result: MOSH provides continuous lateral coverage beyond the native field of view and tunable axial survey ranges up to 20 m, supporting scalable multiscale analysis for biological and clinical specimens.