Identification of an Endothelial Senotype Predicted by Ion Channel Expression in Aged Murine Perigonadal Adipose Tissue

Ion channel transport genes are the most predictive markers of age-dependent senescent endothelial capillary nuclei in aged murine perigonadal adipose tissue, identified by snRNA-Seq.

• Why it matters: Adipose dysfunction in aging is linked to accumulating senescent cells, but specific senescent subtypes and biomarkers remain difficult to identify and characterize.
• What they did: Young and old murine perigonadal adipose were profiled by snRNA-Seq using two independent analyses (SenMayo and SEAC-guideline Pearson correlation) to find senescent nuclei in endothelial clusters.
• The result: Endothelial capillary nuclei overexpressing Mif were identified via subclustering and cell-to-cell communication, and rank analysis linked ion channel transport genes to the senescent nuclei, enabling age-dependent biomarkers.