Detergent lysis controls are critical for single extracellular vesicle phenotyping in SP-IRIS platforms

Detergent-mediated lysis controls reveal that a subset of presumed single-EV events in SP-IRIS/IF persists with both interferometric and fluorescent signals, indicating non-vesicular artifacts.

• Why it matters: Standard single-EV workflows can misinterpret co-isolated non-vesicular particles as authentic EVs because specificity controls are often underreported.
• What they did: The study analyzed human neural secretomes and serum EVs across different SP-IRIS/IF setups, using detergent treatment as a lysis control to test signal specificity.
• The result: Persistence of interferometric and fluorescent signals after detergent treatment indicates inadequate discrimination without detergent-mediated lysis, enabling more reliable single-EV phenotyping on SP-IRIS/IF platforms.