Negative feedback of cyclic di-GMP levels optimizes switching between sessile and motile lifestyles in Vibrio cholerae

Negative feedback regulation of cyclic di-GMP levels via VpsR and CdgC optimizes V. cholerae's transition between motile and biofilm states.

• Why it matters: Balancing motility and biofilm formation is crucial for V. cholerae's pathogenicity, but the mechanisms controlling cyclic di-GMP levels to achieve this balance are not fully understood.
• What they did: The study tracked cyclic di-GMP fluctuations following overexpression of diguanylate cyclase QrgB and its inactive variant, combined with genetic, RNA-seq, and ChIP-seq analyses to identify feedback regulators like VpsR, CdgC, and sRNA SnrC.
• The result: Findings reveal a negative feedback loop involving VpsR-induced CdgC and SnrC that maintains cyclic di-GMP homeostasis, enabling V. cholerae to efficiently switch between lifestyles and enhance infection success.