Benchmarking of Fluorescent Proteins in Trichoderma reesei Using Flow Cytometry.

Benchmarking reveals one yellow and two green fluorescent proteins outperform eGFP in Trichoderma reesei, with a red protein comparable to eGFP for screening applications.

• Why it matters: Limited fluorescent proteins hinder biological research and industrial use of T. reesei, a fungus with high secretion capacity, necessitating expanded tools for its study.
• What they did: Six new fluorescent proteins were tested using an optimized single-copy insertion method replacing the native promoter, enabling accurate comparison of their performance.
• The result: The identified fluorescent proteins improve transformant screening and colabeling in T. reesei, with results not predictable from existing databases, aiding future fungal research.