Genomic alterations and treatment define spatiotemporal patterns of glioblastoma recurrence.

Genomic context and treatment exposure shape glioblastoma recurrence patterns, with TP53 mutations linked to mesenchymal transition and poorer survival in 40 patients.

• Why it matters: Understanding the molecular features of the surgical margin and recurrence is crucial because it influences tumor behavior and patient outcomes, yet remains poorly characterized.
• What they did: The study used histology-guided sampling and single-cell spatial transcriptomics on primary, margin, and recurrent tumors, revealing distinct microenvironments and transcriptomic states across 40 cases.
• The result: Findings show TP53 mutations and sustained proliferation promote a mesenchymal subtype associated with early recurrence and shorter survival, highlighting potential targets for tailored therapies.