CUT&ID for simultaneous profiling of protein-DNA and protein-protein interactions.

CUT&ID enables simultaneous profiling of protein-DNA and protein-protein interactions with high sensitivity from just 1 million cells.

• Why it matters: Understanding the complex interactions between proteins and DNA is crucial for decoding gene regulation, but current methods often require transgenesis or large cell numbers, limiting their applicability.
• What they did: The authors developed a fusion protein combining TurboID, protein A/G, and micrococcal nuclease, allowing in vivo target recognition, biotinylation, and DNA cleavage in a single workflow across various sample types.
• The result: This streamlined approach provides high-quality genomics and proteomics data, broad applicability, and efficient dual readouts, offering a powerful tool for studying gene regulation mechanisms.