Aleurone layer multi-omics reveals constitutive enzyme-inhibitor imbalance in LMA-contrasting wheat cultivars.

Constitutive enzyme-inhibitor imbalance in wheat aleurone underpins LMA susceptibility, with Hartog showing higher inhibitor-to-amylase ratios than Seri.

• Why it matters: Understanding the molecular basis of LMA is crucial because it affects wheat grain quality and economic value, yet the proteome and metabolome of aleurone layers across cultivars remain poorly characterized.
• What they did: Multi-omics analysis was performed on aleurone tissue from three wheat cultivars with contrasting LMA phenotypes—Seri, Kennedy, and Hartog—under different hormone treatments, revealing that cultivar identity explained most proteome variance.
• The result: Findings suggest that LMA susceptibility involves a broader enzyme-inhibitor imbalance rather than elevated amylase alone, providing a molecular framework for breeding resistant wheat cultivars.