A Molecularly Anchored Spatial Transcriptomic Framework for Precise CA1-Subiculum Parcellation and Region-Resolved Analysis in Alzheimer's Disease.
- Open access
A molecularly anchored spatial transcriptomic framework accurately delineates CA1 and Subiculum regions, revealing distinct molecular features and transition zones in the human hippocampus.
- Why it matters: Precise regional annotation of the hippocampal interface is crucial for understanding its role in diseases like Alzheimer's, but traditional methods lack reproducibility and clarity.
- What they did: Using high-definition spatial transcriptomics and single-nucleus RNA sequencing from 12 human donors, the study identified regional markers such as FN1 and ETV1 to define CA1-Sub boundaries and transitions.
- The result: The framework enables consistent, region-specific analysis across datasets and disease states, uncovering transcriptional alterations like mitochondrial metabolism changes in Alzheimer's, advancing hippocampal research and diagnostics.