Outer membrane proteins mediate unconventional secretion of Pseudomonas peroxidase through crosstalk between the Sec pathway and outer membrane vesicles.

Outer membrane proteins facilitate unconventional secretion of DypB2985 in Pseudomonas putida via crosstalk between Sec pathway and OMVs.

• Why it matters: Understanding non-classical secretion mechanisms is crucial for revealing bacterial survival strategies and developing biotechnological applications, as many proteins lack traditional signal peptides.
• What they did: The study examined how OM proteins Lpp1528 and OmpW4836 mediate DypB2985 secretion, using molecular interactions and vesicle incorporation assays to elucidate the process.
• The result: Findings demonstrate that OM proteins act as molecular tethers, enabling stepwise secretion of non-canonical proteins, which broadens knowledge of bacterial secretion systems and offers new biotechnological opportunities.