Mammalian metaphase kinetochores are elastic and require condensin for robust structure and function.

Mammalian kinetochores are highly elastic structures that depend on condensin for stability and proper function under spindle forces, with large-scale deformations observed during metaphase.

• Why it matters: Understanding kinetochore mechanics is crucial because they must withstand and adapt to forces during chromosome segregation, yet their structural dynamics and stability mechanisms are not fully understood.
• What they did: Using super-resolution microscopy and microneedle pulling, the study examined 1 μm-scale deformations and elasticity of kinetochores in mammalian cells, analyzing the role of condensin and protein linkages.
• The result: Findings reveal kinetochores are elastic and require condensin for stable microtubule attachments, with a model suggesting stiff protein linkages support a flexible overall structure to maintain function under force.