Myosin1D directs circumferential F-actin flow to define cell chirality.

Myo1D directs clockwise circumferential F-actin flow, establishing cell chirality in Drosophila macrophages with a key role in organ asymmetry.

• Why it matters: Understanding how intrinsic cell chirality forms is crucial for revealing mechanisms behind left-right asymmetric development, a fundamental aspect of organismal biology.
• What they did: The study examined Myo1D, Myo1C, and Myo2 in Drosophila macrophages, using in vivo observations and in vitro motility assays to analyze their effects on F-actin flow and organization.
• The result: Myo1D organizes F-actin into clockwise-rotating rings, providing a molecular basis for cell and organ chirality, and enabling insights into the mechanisms driving asymmetrical morphogenesis.