Mitotic single-stranded DNA suppression by DDIAS.

DDIAS suppresses mitotic single-stranded DNA and promotes chromosome stability in HR-deficient human cells, with a critical role in genome integrity.

• Why it matters: Understanding how cells maintain genome stability during mitosis is vital, especially in HR-deficient tumors that are prone to genomic instability and therapy resistance.
• What they did: The study identified DDIAS as a DNA-binding effector interacting with TOPBP1, and used inactivation experiments to reveal its role in suppressing mitotic ssDNA and facilitating DNA repair.
• The result: DDIAS enables HR-deficient cells to tolerate genomic instability by promoting mitotic DNA synthesis and accurate chromosome segregation, highlighting its potential as a therapeutic target.