A chemigenetic endogenous Ras activity biosensor for live-cell pharmacology.
A chemigenetic HaloRasAR biosensor enables precise live-cell tracking of endogenous Ras activity with subcellular resolution.
- Why it matters: Understanding Ras signaling dynamics is crucial for cancer research and targeted therapies, but current biosensors lack quantitative accuracy and spatial detail.
- What they did: The team developed HaloRasAR, a biosensor using circularly permuted HaloTag labeled with JF 635, to measure Ras activity in live cells under various stimuli and inhibitors.
- The result: HaloRasAR revealed detailed spatiotemporal Ras activity patterns and subcellular-specific responses to inhibitors, advancing live-cell pharmacology and Ras signaling studies.