ECLIPSE: exploring the dark proteome of ESKAPE pathogens through the sequence similarity network of the Protein Universe Atlas.
- 1 cites
ECLIPSE uncovers 120,985 dark proteins in the ESKAPE pathogens' proteomes, revealing conserved, structurally characterized targets for combating antimicrobial resistance.
- Why it matters: Understanding unannotated proteins in ESKAPE pathogens is critical because many remain functionally unknown, hindering the development of new antimicrobial strategies amid rising resistance.
- What they did: The team developed ECLIPSE, a network-based computational framework that integrates pathogen proteomes into the Protein Universe Atlas, identifying and prioritizing dark protein families through a multidimensional scoring system.
- The result: This approach highlights evolutionarily conserved, structurally defined dark proteins, such as a beta-barrel DUF1302 family member, providing promising new targets for experimental antimicrobial research.