Volume EM reveals desmosome three-dimensional architecture in epithelial cells and tissue models.

Three-dimensional imaging uncovers novel structural features and variability in desmosomes across epithelial and tissue models, including gaps within the outer dense plaque.

• Why it matters: Understanding desmosome architecture is crucial for insights into tissue integrity and disease mechanisms, yet previous knowledge was limited to two-dimensional images, missing key structural details.
• What they did: Using focused ion beam scanning electron microscopy, the study analyzed 3D ultrastructure of desmosomes in epidermoid carcinoma and mammary epithelial cells, comparing homotypic and heterotypic junctions, and quantifying size, shape, and organization.
• The result: Findings reveal structural variability and novel features like gaps, providing a comprehensive 3D description that advances understanding of desmosome function and highlights areas for further investigation in morphogenesis and disease.