Ultrarapid deep 3D histology enables intraoperative mapping of glioma infiltration.
ULTRA achieves rapid, high-resolution 3D histology of glioma tissue within 30 minutes, enabling intraoperative mapping of tumor infiltration at single-cell resolution.
- Why it matters: Accurate intraoperative assessment of glioma margins is crucial for effective surgery, but existing methods are too slow and limited in tissue coverage, risking incomplete tumor removal.
- What they did: The team developed ULTRA, a stain-free, fixation-free platform combining stimulated Raman scattering microscopy with a one-step tissue clearing protocol and AI algorithms to produce detailed 3D histology.
- The result: ULTRA accurately visualizes tumor infiltration and histological features in human glioma samples, providing surgeons with comprehensive, real-time information to improve surgical outcomes.