Multiobjective V(H)H discovery through integrated high-throughput screening and AlphaFold3-guided structural prioritization.

Integrated high-throughput screening and AlphaFold3 structure prediction identify polyspecific V H H antibodies with broad toxin neutralization potential, achieving high-precision structural triage.

• Why it matters: Developing broadly neutralizing therapeutic antibodies is challenging due to the diversity of targets and resource-intensive screening processes, especially for snakebite antivenoms.
• What they did: The team combined yeast display screening, deep sequencing, and AlphaFold3 modeling to discover and structurally prioritize V H H antibodies that bind conserved toxin epitopes across multiple snake species, confirming their blocking activity.
• The result: This approach enables efficient identification and optimization of broadly neutralizing antibodies, reducing experimental screening efforts and providing a versatile framework for targeting highly variable antigens.