A conserved β-sheet motif mediates PIN2 hydrophilic loop-loop interactions to regulate PIN2 dynamics.
A conserved β-sheet motif in PIN2's hydrophilic loop is essential for its dynamic localization and function, with deletion causing clustering and trafficking defects in Arabidopsis.
- Why it matters: Understanding PIN2 regulation is crucial because its proper localization controls plant development processes like root gravitropism, yet the structural mechanisms behind its regulation are unclear.
- What they did: The study identified a conserved β-sheet motif mediating homotypic PIN2-HL interactions, using deletion mutants and rapamycin-induced reconstitution to demonstrate its role in PIN2 dynamics and trafficking.
- The result: Disrupting this motif impairs PIN2 function, but restoring HL-HL interactions rescues normal behavior, highlighting this motif as a regulatory hub influenced by phosphorylation that modulates PIN2 activity.