Single-neuron connectome of nucleus accumbens.

Single-neuron connectivity patterns in the nucleus accumbens reveal distinct input and output organization, with topographic distribution and subtype-specific projections in mice.

• Why it matters: Understanding the detailed wiring of NAc neurons is crucial for deciphering the neural circuits underlying reward and motivation, yet this connectivity at the single-cell level has been poorly characterized.
• What they did: The study used rabies virus tracing and single-cell resolution analysis to map input and projection patterns of medium spiny neurons, identifying differences between D1 and D2 subtypes and their cortical and hippocampal connections.
• The result: Findings highlight specific topographic and collateralization patterns, providing an anatomical framework that advances understanding of NAc circuit mechanisms and their roles in behavior.