eVLP compound delivery breaks the prime editing efficiency ceiling.

Repeated dosing with engineered virus-like particles enhances prime editing efficiency beyond previous limits.

• Why it matters: Efficient and reproducible delivery of prime editing machinery is crucial for large-scale gene variant analysis, but current methods face limitations in effectiveness and consistency.
• What they did: The authors developed PRIME-VLP, a strategy involving repeated doses of engineered virus-like particles, to optimize the timing and delivery of prime editors.
• The result: This approach significantly improves editing efficiency and screening performance, enabling more effective gene editing applications and advancing the potential of prime editing technology.