Recent developments of fluorescent proteins and biosensors driven by cross-disciplinary insights.

Recent advances in fluorescent proteins and biosensors have significantly improved photostability, wavelength range, and multiplexing capabilities, exemplified by mScarlet.

• Why it matters: Enhancing fluorescent proteins and biosensors is crucial for more precise and versatile biological imaging, addressing limitations in stability, resolution, and signal differentiation.
• What they did: The authors review recent developments in FP engineering and biosensor design, focusing on improvements driven by cross-disciplinary insights from chemistry, physics, engineering, and screening technologies.
• The result: These innovations enable more durable, brighter, and multiplexed imaging tools, paving the way for future FPs and biosensors with expanded applications in biological research.