Liver X receptors participate in the fasting response by assisting CEBPB binding to enhancers and promoting the fasting transcriptional program.

Double knockout of liver X receptors impairs fasting-induced gene activation and enhancer accessibility in the liver.

• Why it matters: Understanding how transcription factors regulate fasting responses is crucial for insights into metabolic homeostasis, yet the role of LXRs in fasting was previously unclear.
• What they did: The study used transcriptome and chromatin accessibility profiling in single- and double-knockout mice lacking LXR isoforms, combined with ChIP-seq for NR1H3, to examine their roles during fasting.
• The result: Findings show LXRs directly bind enhancers, increase their accessibility, assist CEBPB binding, and promote fasting-induced gene expression, revealing LXRs as novel regulators of the fasting response.